Journal: BMC Musculoskeletal Disorders

Article Title: High molecular weight hyaluronic acid alleviates ovariectomy-induced bone loss in mice

doi: 10.1186/s12891-024-08161-y

Figure Lengend Snippet: HMW-HA downregulated osteoclastic bone resorption in ovariectomized mice through the downregulation of RANKL-expressing osteocytes. A The area analyzed for osteoclast parameters is indicated in the box in yellow. B Representative images of TRAP staining. Low-power images boxed in red are enlarged and presented in each panel. Black arrows indicate osteoclasts. Red bars in high-power images indicate scale bars (50 μm). C Bone resorption parameters including osteoclast surface per bone surface (Oc.S/BS) and osteoclast number per bone surface (N.Oc/BS) of each sagittal section. D The area analyzed for Rankl + osteocyte percentage is indicated in the box in green. E Representative images of RANKL-positive osteocyte lacunes. Low-power images boxed in red are enlarged and presented in each panel. Black arrows indicate RANKL-positive osteocyte lacunes. Red arrows indicate osteocytes with no RANKL expression. Red bars in high-power images indicate scale bars (50 μm). F The Rankl. + osteocyte percentage was qualitatively assessed in each group. Data are expressed as mean ± SD, analyzed by ANOVA followed by Tukey’s post hoc test, showing all data points. * p < 0.05, ** p < 0.01, *** p < 0.001. The number of samples are indicated at the bottom of each figure and in Supplementary Tables 3 and 4

Article Snippet: Rabbit anti-mouse RANKL polyclonal antibody (cat#GTX32834) was purchased from Funakoshi (Tokyo, Japan).

Techniques: Expressing, Staining

Journal: BMC Musculoskeletal Disorders

Article Title: High molecular weight hyaluronic acid alleviates ovariectomy-induced bone loss in mice

doi: 10.1186/s12891-024-08161-y

Figure Lengend Snippet: Removal of HMW-HA from estrogen-deficient culture medium increased the RANKL expression in ST2 cells. A Experimental plan. Three hundred thousand cells were incubated for 12 h in RPMI-1640 medium. After cells were attached to the dishes, culture media were replaced with (I) charcoal-stripped FBS and (II) charcoal-stripped FBS with 100 nM of hyaluronidase for 12 h or 24 h. B , C Relative expression of RANKL and OPG in the presence or absence of hyaluronidase at 12 and 24 h. Colorful lines are associated with each 12 and 24 h samples. D Average ratio of RANKL mRNA to OPG mRNA in the presence or absence of hyaluronidase. Data are expressed as mean ± SD, analyzed by Wilcoxon signed-rank test ( B , C ), Wilcoxon rank-sum test ( D ), showing all data points. * p < 0.05. ns, not significant. n = 6 per group. Numerical data are presented in Supplementary Table 6

Article Snippet: Rabbit anti-mouse RANKL polyclonal antibody (cat#GTX32834) was purchased from Funakoshi (Tokyo, Japan).

Techniques: Expressing, Incubation